The first Interdisciplinary Summer School Chemical and Physical Probes for Biology 2023: how to spy on cell dynamics?  (ChemPhysBio2023) is an annual thematic school organized by the Institute for the Sciences of Light and BioProbe of Paris-Saclay University.

Course overview

The fine understanding of molecular mechanisms in native biological systems is an important step in rationalizing, preventing and ultimately curing diseases. Photonic imaging plays an important role in this field. Quantitative imaging experiments designed to answer complex biological questions require the implementation of efficient probes and adapted imaging setups and data processing workflows. This interdisciplinary school will provide an overview of these different chemical, physical and biological aspects. It will offer also interactive and interdisciplinary workshops to learn how to communicate effectively between disciplines as well as two half-days of hands-on.

Audience

This high-level training opportunity is aimed at newcomers to the field, master and doctoral students, post-doctoral fellows and junior researchers, giving them the opportunity to discover the research laboratories of the Université Paris-Saclay working in this discipline. 20 interdisciplinary trainees will be selected on the basis of their motivation.

Learning outcomes

Participants will gain an overview of strategies for quantitative, spatial, and temporal exploration of biological events: from the biological question to probes and instruments. Courses are divided into basic lectures, more advanced courses, and research seminars.

Participants will develop their ability to collaborate and communicate with colleagues from other disciplines to initiate and conduct interdisciplinary projects.

Lectures:

• Optical Microsopy (G. Dupuis, ISMO, Univ Paris Saclay)
• Biology from cells and tissus to biomolecules (O. Nüsse, ICP, Univ Paris Saclay)
• Photophysics: interaction of light with matter (H. Pasquier, ICP, Univ Paris Saclay)
• Relationship between Structure&Reactivity from molecule to the probe (1&2) (A. Romieu, ICMUB, Univ de Bourgogne)
• SuperResolution Imaging (S. Leveque-Fort, ISMO, Univ. Paris Saclay)
• Coupling microfluidic and microscopy to explore living matter (A. Pallandre, ICP, Univ Paris Saclay)
• Quantitative imaging of cell chemistry with biosensors, FRET modalities (1&2) (F. Riquet, PhLAM, Univ de Lille)
• Single molecule tracking and applications (F. Marquier, LuMIn, ENS Paris-Saclay)
• Click Chemistry / in cell macromolecules labelling (B. Vauzeilles, ICSN, CNRS)
• Electron microscopy and correlative imaging, perspective (E. Quemin, I2BC, CNRS)

Hands-on:

• Detection of FRET by FLIM to probe protein interactions at membrane contact sites (D. Zamiati)
• Dynamic analysis of lipids in cell membranes (S. Dupré)
• Proteins recruitment after DNA damages on living cells, imaging and analysis (M.-N. Soler)
• Measuring bacteria internal pH using PHP biosensor and light microscopy  (R. Le Bars)
• Super-resolution imaging (G. Dupuis)
• Monitoring of intracellular pH with a ratiometric biosensor in living cells (M. Bourge)
• Microfluidic and optical detection (A. Pallandre)
• Characterization of spectroscopic properties of a probe (M. Erard)
• Investigation of endolplasmic reticulum – mitochondria contact sites using structured illumination microscopy (S.I.M.) (S. Lecart)
• 2D single-particle tracking using Total Internal Reflection Fluorescence Microscopy (K. Perronet)

• Arnaud Chevalier (ICSN)

• Sophie Dupré (ICP)

• Oliver Nüsse (ICP)

• Karen Perronet (LuMIn)

• Alexandra Fragola (ISMO)

• Dominique Guianvarch (ICMMO)

• Antoine Pallandre (ICP)

• Boris Vauzeilles (ICSN)

• Cécile Bouton (ICP)

• Nina Tyminska (ISL)

• Fabienne Goldfarb (ISL)

• Marie Erard (BioProbe)

We thank you for your generous support!